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KMID : 0352719920160030210
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Journal of Ginseng Research
1992 Volume.16 No. 3 p.210 ~ p.216
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Effects of Ginseng Saponin on DNA Strand Breaks and Replication Inhibition by Benzo(a)Pyrene in CHO-K1 Cells
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Abstract
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The effect of saponin extracted from Panax ginseng C. A. Meyer on DNA repair and replicative DNA synthesis were examined in CHO-K1 cells cotreated with benzo(a)pyrene and rat liver S-15 fraction. The DNA strand breaks inititated by benzo(a)pyrene
metabolites were measured by alkaline elution technique, The addition of ginseng saponin to the culture media resulted in decrease of benzo(a)pyrene-induced DNA strand breaks, and restored the suppressed-semiconservative-DNA-synthesis by the
carcinogen.
DNA repair synthesis in the damaged cells was also elevated by the ginseng treatment when the repairing activites were measured for the [3H]-thymidine incorporation into the carcinogen damaged cellular DNA. Comparative analysis of DNA-adducts of
benzo(a)pyrene metabolites in microsomes suggested that ginseng saponin treatment in rats reduced the formation of electrophilic metabolites of benzo(a)-pyrene in the rat liver microsomes.
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